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SIRT1 is redistributed at the chromatin level, causing deregulation at the transcription level and DNA damage (Oberdoerffer et al., 2008)

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The success of such surface-based conjugation hinges on two critical criteria: (i) the availability of orthogonal chemical handles that selectively target specific amino acid residues without cross-reacting with other surface-exposed side chains, and (ii) coupling chemistries that preserve native protein folding and function